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Study of Protein Hydrodynamics with Light Scatter-ing: Size and Charge of Lysozyme
閱讀：215 發(fā)布時(shí)間：2015-3-12| 提 供 商 | 美國(guó)布魯克海文儀器公司 | 資料大小 | 451.8KB |
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Effective determination of the size and charge status of biological macromolecules like proteins plays a crucial role in pharmaceutical, clinical and other biotechnological applications. In the present study, dynamic light scattering (DLS) and electrophoretic light scattering (ELS) are employed to characterize the size and charge of Lysozyme, a common protein. It is shown that the sensitivity and precision of these techniques are well suitable to discern the subtle effects of buffer solution conditions on the protein size and charge.