Recombinant human Keratinocyte Growth Factor is a disulfide-linked monomeric protein consisting of 164 amino acid residues. Optimized DNA sequence encoding Human Keratinocyte Growth Factor mature chain was expressed in E. coli. Native human KGF is generated by the proteolytic removal of the signal peptide and propeptide. KGF migrates as an approximately 19 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.