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BPS Bioscience Inc.
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CBL-B-Driven Tyro3 Ubiquitination Intrachain TR-FRET Assay Kit

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產品型號78388

品       牌BPS Bioscience

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更新時間:2023-11-12 22:55:26瀏覽次數:173次

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●Purchase●DescriptionTheCBL-B-drivenTyro3UbiquitinationIntrachainTR-FRETAssayKitisasensitivehigh-throughputscreening(HTS)TR-FRETAssayKit
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Description

The CBL-B-driven Tyro3 Ubiquitination Intrachain TR-FRET Assay Kit is a sensitive high-throughput screening (HTS) TR-FRET Assay Kit, designed to measure CBL-B E3 ligase activity in a homogeneous 384 reaction format. It utilizes a Europium-labeled Ubiquitin (donor) and a Cy5-labeled Ubiquitin (acceptor) to complete the TR-FRET pairing. Since both the TR-FRET donor and acceptor are incorporated into poly-ubiquitin chains, this FRET-based assay requires no time-consuming washing steps, making it especially suitable for HTS applications as well as real-time kinetics analyses of polyubiquitination. Of note, the assay kit does not detect mono-ubiquitination.

Alternatively, under the same experimental conditions the AXL kinase (BPS Bioscience #40180) can be used instead of Tyro3 as C-CBL ubiquitination substrate.

CBL-B-driven Tyro3 Ubiquitination Intrachain TR-FRET

Figure 1. CBL-B-driven Tyro3 ubiquitination intrachain TR-FRET Assay Kit schematic

Synonyms
Tyrosine-protein kinase receptor, TYRO3, BYK, DTK, RSE, SKY, TIF, E3 ubiquitin-protein ligase CBL-B, Casitas B-lineage lymphoma proto-oncogene b, RING finger protein 56, RING-type E3 ubiquitin transferase CBL-B, SH3-binding protein CBL-B

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