The DNMT3B Direct Activity Assay Kit is designed to measure DNMT3B activity for screening and profiling applications. The key to the DNMT3B Direct Activity Assay Kit is a highly specific antibody that recognizes 5-methylcytosine of the substrate. With this kit, only three simple steps on a microtiter plate are required for detection of DNMT3B activity. First, S-adenosylmethionine is incubated with a sample containing assay buffer and DNMT3B/3L for two hours. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

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