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BPS Bioscience Inc.
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當(dāng)前位置:BPS Bioscience Inc.>>PARP Assay Kits>> 80560PARP10 Chemiluminescent Assay Kit

PARP10 Chemiluminescent Assay Kit

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產(chǎn)品型號(hào)80560

品       牌BPS Bioscience

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更新時(shí)間:2023-11-12 08:29:00瀏覽次數(shù):212次

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●Purchase●DescriptionThePARP10ChemiluminescentAssayKitisdesignedtomeasurePARP10activityforscreeningandprofilingapplications
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Description

The PARP10 Chemiluminescent Assay Kit is designed to measure PARP10 activity for screening and profiling applications. PARP10 is known to catalyze the NAD-dependent ADP-ribosylation of histones. The key to the PARP10 Chemiluminescent Assay Kit is the biotinylated NAD+. With this kit, only three simple steps are required for PARP10 reactions. First, histone proteins are coated on a 96-well plate. Next, a biotinylated NAD+ mix (termed PARP Substrate Mixture) is incubated with the PARP10 enzyme in an optimized assay buffer. Finally, the plate is treated with streptavidin-HRP followed by addition of the ELISA ECL substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.

PARP10  Schematic

Figure 1. PARP10 Chemiluminescent Assay Kit schematic

*NOTE: As of April 2022, this protocol has been re-optimized for performance. Previous versions of this kit are available upon request.

This product has been cited 1 time.

Synonyms
poly (ADP-ribose) polymerase 10, poly(ADP-ribosyl)transferase 10, PARP10

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