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PARP7 Chemiluminescent Assay Kit

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產品型號79729

品       牌BPS Bioscience

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更新時間:2023-11-12 08:11:56瀏覽次數:200次

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●Purchase●DescriptionThePARP7ChemiluminescentAssayKitisdesignedtomeasurePARP7activityforscreeningandprofilingapplications
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Description

The PARP7 Chemiluminescent Assay Kit is designed to measure PARP7 activity for screening and profiling applications. PARP7 is known to catalyze the NAD-dependent ADP-ribosylation of histones. The key to the PARP7 Chemiluminescent Assay Kit is the biotinylated NAD+. With this kit, only three simple steps are required for PARP7 reactions. First, histone proteins are coated on a 96-well or 384-well plate. Next, a biotinylated NAD+ mix (termed PARP Substrate Mixture) is incubated with the PARP7 enzyme in an optimized assay buffer. Finally, the plate is treated with streptavidin-HRP followed by addition of the ELISA ECL substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.

PARP7 Chemiluminescent Assay Kit

Figure 1. PARP7 Chemiluminescent Assay Kit schematic

*NOTE: As of April 2022, this protocol has been re-optimized for performance. Previous versions of this kit are available upon request.

Synonyms
TCDD Inducible Poly(ADP-Ribose) Polymerase, ADP-Ribosyltransferase Diphtheria Toxin-Like 14,TCDD-Inducible Poly [ADP-Ribose] Polymerase, Poly [ADP-Ribose] Polymerase

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