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BPS Bioscience Inc.
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TNKS2 (PARP5B) Colorimetric Assay Kit

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產(chǎn)品型號78577

品       牌BPS Bioscience

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更新時間:2023-11-11 23:52:36瀏覽次數(shù):157次

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●Purchase●DescriptionTheTNKS2ColorimetricAssayKitisdesignedtomeasuretheactivityofTankyrase2(TNKS2,alsoknownasPARP5B)forscreeningandprofilingapplications
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Description

The TNKS2 Colorimetric Assay Kit is designed to measure the activity of Tankyrase 2 (TNKS2, also known as PARP5B) for screening and profiling applications. TNKS2 is known to catalyze the NAD-dependent ADP-ribosylation of histones. The key to the TNKS2 Colorimetric Assay Kit is the biotinylated NAD+. First, histone proteins are coated on a 96-well plate. Next, a biotinylated NAD+ mix (termed PARP Substrate Mixture) is incubated with the TNKS2 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the colorimetric HRP substrate to produce color that can be measured using a UV/Vis spectrophotometer microplate reader.

Figure 1. TNKS2 (PARP5B) Colorimetric Assay Kit schematic

*NOTE: As of March 2023, this protocol has been re-optimized for performance. Previous versions of this kit are available upon request.

Synonyms
Tankyrase II, Tankyrase-2, TANK2, Tankyrase-like protein, Tankyrase-related protein, TNKL, Poly [ADP-ribose] polymerase tankyrase-2, ADP-ribosyltransferase diphtheria toxin-like 6, ARTD6, Poly [ADP-ribose] polymerase 5B, Protein poly-ADP-ribosyltransferas

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