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BPS Bioscience Inc.
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TNKS2 (PARP5B) Chemiluminescent Assay Kit

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產品型號78406

品       牌BPS Bioscience

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更新時間:2023-11-11 23:50:51瀏覽次數:186次

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●Purchase●DescriptionTheTNKS2ChemiluminescentAssayKitisdesignedtomeasuretheactivityofTankyrase2(TNKS2,alsoknownasPARP5B)forscreeningandprofilingapplications
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Description

The TNKS2 Chemiluminescent Assay Kit is designed to measure the activity of Tankyrase 2 (TNKS2, also known as PARP5B) for screening and profiling applications. TNKS2 is known to catalyze the NAD-dependent ADP-ribosylation of histones. The key to the TNKS2 Chemiluminescent Assay Kit is the biotinylated NAD+. With this kit, only three simple steps are required for TNKS2 reactions. First, histone proteins are coated on a 96-well or 384-well plate. Next, a biotinylated NAD+ mix (termed PARP Substrate Mixture) is incubated with the TNKS2 enzyme in an optimized assay buffer. Finally, the plate is treated with streptavidin-HRP followed by addition of the ELISA ECL substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.

TNKS2 PARP5B Chemiluminescent

Figure 1. TNKS2 (PARP5B) Chemiluminescent Assay Kit schematic

*NOTE: As of April 2022, this protocol has been re-optimized for performance. Previous versions of this kit are available upon request.

Synonyms
Poly [ADP-ribose] polymerase tankyrase-2, ADP-ribosyltransferase diphtheria toxin-like 6, ARTD6, Poly [ADP-ribose] polymerase 5B, Protein poly-ADP-ribosyltransferase tankyrase-2, TNKS-2, TRF1-interacting ankyrin-related ADP-ribose polymerase 2, Tankyrase

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