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BPS Bioscience Inc.
中級會員 | 第3年

當前位置:BPS Bioscience Inc.>>Immunotherapy Cell Lines>> 78757CD8+ TCR Knockout NFAT-Luciferase Reporter Jurkat Cell Line

CD8+ TCR Knockout NFAT-Luciferase Reporter Jurkat Cell Line

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產品型號78757

品       牌BPS Bioscience

廠商性質生產商

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更新時間:2023-11-11 15:51:39瀏覽次數:266次

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●Purchase●DescriptionThiscelllinewasgeneratedfromTCRKnockoutNFATLuciferaseReporterJurkatCellLine(BPSBioscience#78556)byexogeneousoverexpressionofhumanCD8(NM_001768
Purchase
Description

This cell line was generated from TCR Knockout NFAT Luciferase Reporter Jurkat Cell Line (BPS Bioscience #78556) by exogeneous overexpression of human CD8 (NM_001768.6) using lentiviral transduction (CD8a Lentivirus, BPS Bioscience #78648). To achieve knockout of TCR (T Cell Receptor), the TRAC (T-Cell Receptor Alpha Constant) and TRBC1 (T-Cell Receptor Beta Constant 1) domains of the TCRα/β chains were genetically removed by CRISPR/Cas9 genome editing from recombinant Jurkat cells stably expressing the firefly luciferase gene under the control of NFAT response elements.

TCRα/β knockout in the TCR Knockout NFAT-Luciferase Reporter Jurkat cells was confirmed by genomic sequencing and by flow cytometry and expression of CD8 was confirmed by flow cytometry. The cell line has been functionally validated and does not respond to anti-CD3 agonist antibodies, as opposed to parental NFAT-Luciferase Reporter Jurkat cells (BPS Bioscience #60621).

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

Synonyms
T-lymphocyte differentiation antigen T8/Leu-2, CD8a, MAL

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