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BPS Bioscience Inc.
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NF-κB Reporter Cellular Assay Pack (HCT116)

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更新時間:2023-10-12 09:44:00瀏覽次數:173次

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  • 數量:

    1

  • 供應商:

    BPS Bioscience

  • 細胞類型:

    穩轉細胞系

  • 品系:

    HCT116

  • 組織來源:

    /

  • 相關疾病:

    多種

  • 物種來源:

    human

  • 免疫類型:

    多種

  • 器官來源:

  • 運輸方式:

    干冰

  • 年限:

    10

  • 生長狀態:

    良好

  • CAS號:

  • 英文名:

    NF-κB Reporter Cellular Assay Pack (HCT116)

  • 注冊證號:

    /

  • 規格:

    2管

The NF-κB Reporter Cellular Assay Pack provides all the key reagents required to monitor the activity of the nuclear factor Kappa B (NF-κB) signal transduction pathways. The pack contains the NF-κB Reporter (Luc)- HCT-116 Recombinant Cell Line, a luciferase reporter cell line that contains a firefly luciferase gene under the control of four copies of the NF-κB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or stimulants of lymphokine receptors, endogenous NF-κB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene. This cell line is validated for the response to TNFalpha and to treatment with NF-κB inhibitor, evodiamine.Additionally, the pack includes cell culture medium (Thaw Medium 7) that has been optimized for use with HCT-116 cells. Thaw Medium 7 includes 10% fetal bovine serum and 1% Pen/Strep. Finally, the pack provides the ONE-Step™ Luciferase Detection System. This reagent provides highly sensitive, stable detection of firefly (Photinus pyralis) luciferase activity. The ONE-Step luciferase reagent can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required.

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