The PRMT1 Direct Activity Chemiluminescent Assay kit is designed to measure PRMT1 activity for screening and profiling applications. The key to the PRMT1Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated R3residue of Histone H4. With this kit, only three simple steps are required formethyltransferase detection. First, S-adenosylmethionine is incubated with a samplecontaining assay buffer and methyltransferase enzyme. Next, primary antibody is added.Finally, the strip plates are treated with an HRP-labeled secondary antibody followed byaddition of the HRP substrate to produce chemiluminescence that can then be measuredusing a chemiluminescence reader.