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BPS Bioscience Inc.
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Spike (SARS-CoV-1) Pseudotyped Lentivirus (eGFP Reporter)

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更新時間:2023-10-11 11:26:36瀏覽次數:248次

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保存條件:-80°C(dryice)保質期:3-18month英文名:Spike(SARS-CoV-1)PseudotypedLentivirus(eGFPReporter)數量:1供應商:BPSBioscienceInc
  • 保存條件:

    -80°C (dry ice)

  • 保質期:

    3-18 month

  • 英文名:

    Spike (SARS-CoV-1) Pseudotyped Lentivirus (eGFP Reporter)

  • 數量:

    1

  • 供應商:

    BPS Bioscience Inc.

  • CAS號:

    /

  • 規格:

    100 µl

Severe acute respiratory syndrome (SARS) was the first new infectious disease identified in the twenty-first century. It is a viral respiratory disease caused by severe acute respiratory syndrome coronavirus (SARS-CoV-1). The first known cases occurred in November 2002, and these viral infections led to the 2002-2004 SARS outbreak. Since 2004, no cases of SARS-CoV-1 have been reported worldwide. A virus very similar to SARS-CoV-1 was discovered in late 2019. This virus, named severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is the causative pathogen of , the spread of which started the pandemic.SARS-CoV-1 attaches to the host cell surface before entering the cell. The Spike protein on the virus recognizes and binds to the Angiotensin-Converting Enzyme 2 (ACE2) receptor found on the surface of human airway epithelia as well as lung parenchyma. Drugs targeting the interaction between the Spike protein of SARS-CoV-1 and ACE2 may offer protection against the viral infection.The Spike (SARS-CoV-1) Pseudotyped Lentiviruses were produced with SARS-CoV-1 Spike (Genbank Accession #YP_009825051.1) as the envelope glycoproteins instead of the commonly used VSV-G. These pseudovirions contain the eGFP gene driven by a CMV promoter, therefore, the spike-mediated cell entry can be determined via eGFP fluorescence. The Spike (SARS-CoV-1) pseudotyped lentivirus can be used to measure the activity of a neutralizing antibody against SARS-CoV-1 in a cellular context, using a Biosafety Level 2 facility.The Spike (SARS-CoV-1) pseudovirus has been validated for use with ACE2-HEK293 target cells (which overexpress ACE2; BPS Bioscience #79951).
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