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BPS Bioscience Inc.
中級會員 | 第3年

13829799985

當前位置:BPS Bioscience Inc.>>試劑>>克隆與表達>> AAV1 SaCas9

AAV1 SaCas9

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品       牌BPS Bioscience

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更新時間:2024-11-23 12:08:26瀏覽次數(shù):177次

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保存條件:-80°C(dryice)保質期:3-18month英文名:AAV1SaCas9數(shù)量:1供應商:BPSBioscienceInc
  • 保存條件:

    -80°C (dry ice)

  • 保質期:

    3-18 month

  • 英文名:

    AAV1 SaCas9

  • 數(shù)量:

    1

  • 供應商:

    BPS Bioscience Inc.

  • CAS號:

    /

  • 規(guī)格:

    50 µl x 2

Adeno-Associated Virus Serotype 1 (AAV1) exhibits high homology with other AAV serotypes. AAV1 efficiently transduces muscle tissue, as determined by a region of the capsid protein VP1 (amino acids 350 to 430) which functions as a major determinant of tissue tropism.Cas9 is an endonuclease enzyme that introduces a double stranded break into the DNA when recruited to a specific DNA sequence by the sgRNA (single guide RNA). This double stranded break is repaired in mammalian cells either through Non-Homologous End Joining or Homologous Recombination. Non-Homologous End Joining often results in the deletion or insertion of several base pairs at the cut site, which, when resulting in a frameshift, causes the functional inactivation of the gene.SaCas9 (Staphylococcus aureus CRISPR-associated protein 9) has demonstrated high cutting efficiency in mammalian cells, and its smaller size makes it ideal for packaging into AAV. SaCas9 recognizes a longer protospacer adjacent motif (PAM) site, 5'-NNGRRT-3', than the more traditional SpCas9 (Streptococcus pyogenes CRISPR-associated protein 9). These AAV particles constitutively express SaCas9 under the control of a CMV promoter.
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