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人結(jié)腸癌細胞COLO205 種屬人別稱Colo205;CoLo205;COLO-205;Colo-205; COLO.205; Colo205; COLO205; Co 205; Colorado 205組織來源源自轉(zhuǎn)移部位：腹水疾病結(jié)腸腺癌傳代比例/細胞消化1:2傳代

人結(jié)腸癌細胞COLO205

種屬	人
別稱	Colo 205; CoLo 205; COLO-205; Colo-205; COLO.205; Colo205; COLO205; Co 205; Colorado 205
組織來源	源自轉(zhuǎn)移部位：腹水
疾病	結(jié)腸腺癌
傳代比例/細胞消化	1:2傳代，懸浮部分離心收集(1000RPM,5分鐘) ，貼壁部分消化2-3分鐘
培養(yǎng)基配置	RPMI1640培養(yǎng)基；10%胎牛血清； 1%雙抗
簡介	該細胞系是1957年由T.U.SAMPLE等從患有結(jié)腸癌的70歲男性白人的腹水中分離的。該病人在取腹水樣品前已用5- 氟治療4~6周。角蛋白免疫過氧化物酶染色陽性；產(chǎn)生CEA、IL10
形態(tài)	上皮細胞樣
生長特征	貼壁，懸浮混合生長
倍增時間	~25-30h
基因表達	carcinoembryonic antigen (CEA) 1.5 to 4.1 ng/10^6 cells cells/10 days; keratin; interleukin 10 (IL-10, interleukin-10), The cells are positive for keratin by immunoperoxidase staining.
致瘤性	Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 1×10^7 cells).
STR	Amelogenin: X CSF1PO: 11,12 D13S317: 10,12 D16S539: 12,13 D5S818: 10,13 D7S820: 9,10 THO1: 8,9 TPOX: 11 vWA: 15
培養(yǎng)條件	氣相：空氣，95% ；二氧化碳，5%。溫度：37攝氏度，培養(yǎng)箱濕度為70%-80%。
凍存條件	凍存液：90%FBS ，DMSO 10%, 或使用非程序凍存液：貨號JY-H040
保藏機構(gòu)	ATCC; CCL-222
備注	該細胞為半懸浮和半貼壁細胞，懸浮細胞離心收集，貼壁細胞消化處理

-stimulatory approaches. Effects on the immunophenotype were assessed via flow cytometry and RNA-seq analyses. The changes in RNA-seq-based immune signatures determined in a murine setting were investigated in patient samples from the first-dose cohort of the SENSITIZE trial (NCT03278665) evaluating domatinostat combined with pembrolizumab in advanced-stage

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