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【文獻標題】Silencing of Wnt5a prevents interleukin?1β?induced collagen type II degradation in rat chondrocytes

【作者】SHIPING SHI, ZHENTAO MAN, WEI LI, et.al

【作者單位】山東大學附屬醫院（Shandong Provincial Hospital Affiliated）

【文獻中引用產品】

試劑—氨基甲酸乙酯

【關鍵詞】osteoarthritis, Wnt5a signaling pathway, small interfering RNA, gene silencing, collagen type II

【DOI】10.3892/etm.2016.3788

【影響因子(IF)】2.44

【出版期刊】《EXPERIMENTAL AND THERAPEUTIC MEDICINE》

【產品原文引用】

Articular chondrocyte culture and identification. Chondrocytes  from Sprague-Dawley rats (n=24 rats; age, 9 weeks; weight, 

180±12 g) were harvested according to a previous study (17). These rats were fed with standard laboratory food (containing 

1.56% calcium, 0.8% phosphorus and 800 IU/kg vitamin D) and kept under a 12 h light/dark cycle at 24?C and 50% humidity. After sacrifice with an overdose of anesthetic (12 ml/kg 20% urethane; Hengyuan Biological Technology Co., Ltd., Shanghai, China), the cartilage was sliced into small pieces from the femoral trochlea and digested with 2.0 mg/ml type II collagenase (Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA) at 37?C for 4 h. Then, the digest solution was filtered with 200 mesh?filtrating screen to remove large fragments. 

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