GENERAL INFORMATION

Product Overview

The NEXTFLEX® Rapid DNA-Seq Kit 2.0 is designed for an approximately 3-hour DNA library construction with 1 ng – 1 µg of fragmented DNA. This kit is not recommended for FFPE samples. The kit can be used to prepare multiplexed libraries for single or paired end sequencing using Illumina® platforms. In addition, the availability of up to 1536 unique adapter barcodes facilitates high-throughput applications. There are five main steps involved in preparing DNA for sequencing: DNA extraction, DNA fragmentation, DNA end repair / adenylation, adapter ligation, and PCR amplification. The NEXTFLEX® Rapid 2.0 DNA Seq Kit contains the necessary material to take the user’s purified and fragmented DNA through preparation and amplification for loading onto flow cells for sequencing.

Kit Overview

The NEXTFLEX® Rapid DNA-Seq Kit 2.0 contains enough material to prepare 8, 48, or 96 DNA samples for Illumina® compatible sequencing.

Kit Contents, Storage, and Shelf Life

The shelf life of all reagents is at least 12 months when stored properly. All components should be stored at -20°C, except the Nuclease-free Water and Resuspension Buffer, which can be safely stored at room temperature, and NEXTFLEX® Cleanup Beads 2.0, which should be stored at 4°C.