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Source

Mouse

Applications

WB,IP,IF

Reactivity

Species independent

Dilution

WB: 1:5000; IP: 1:200 IF: 1:2000

Storage

-20°C/1 year

Specificity

The antibody detects C-terminal, internal, and N-terminal Flag-tag fusion proteins.

Source/Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Immunogen

Synthetic Peptide of Flag-Tag

Form

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Clonality

Monoclonal

Background

The DYKDDDDK peptide (Flag-tag) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, and then used to separate recombinant, over expressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

Concentration

1 mg/ml