CD8(T細胞)兔單克隆抗體

廣州健侖生物科技有限公司

CD8作為T細胞受體的共受體，是一種跨膜糖蛋白。在細胞毒性T淋巴細胞/抑制T淋巴細胞呈現高表達，而NK細胞呈現低表達。在成熟T細胞中CD4和CD8常出現互相排斥的表達。所以，CD8常與CD4合用用于研究輔助性T淋巴細胞及其腫瘤，如外周T細胞淋巴瘤（CD4+/CD8-）；間變性大細胞淋巴瘤（CD4+/CD8-)。T淋巴母細胞淋巴瘤CD4和CD8可同時表達。

我司還提供其它進口或國產試劑盒：登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲病、違禁品濫用、肺炎球菌、軍團菌、化妝品檢測、食品安全檢測等試劑盒以及日本生研細菌分型診斷血清、德國SiFin診斷血清、丹麥SSI診斷血清等產品。

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【產品介紹】

細胞定位：細胞膜

克隆號：SP16

同型：IgG1

適用組織：石蠟/冰凍

陽性對照：扁桃體

抗原修復：熱修復（EDTA）

抗體孵育時間：30-60min

CD8(T細胞)兔單克隆抗體

2. 細胞毒試驗
檢測CTL細胞毒作用均可采用檢測NK細胞殺傷活性的方法，僅效靶細胞比例不一樣，現將LDH釋放法簡要敘述如下：
①  靶細胞為用作刺激細胞的自身或同種異體EBV-LCL細胞，調成1×105/ml；
②  效應細胞為上述誘導的特異性CTL，調成2.5×106/ml；
③  各取0.1ml于96孔板中（效/靶比值為25:1）；輕輕吹打，使二者混勻；同時設靶細胞自然釋放對照組（即只加靶細胞而不加效應細胞）和zui大釋放對照組（0.1ml靶細胞和0.1ml 1% NP-40）；
④  1000rpm/min離心2min后，置37°C、5% CO2 培養箱中孵育4hr
⑤  酶促顯色反應：參見“NK細胞殺傷實驗”。
中性粒細胞（polymorphonuclear leukocyte，PMN）的功能包括粘附、移動、吞噬殺菌等，是機體天然免疫力的重要組成部分。
試劑及材料
1. 白色葡萄球菌
2. 肉湯培養基
3. 鹼性美藍液
操作方法
1. 菌液的制備  將白色葡萄球菌接種于中，放37℃溫箱內培養12h左右；置水浴中加熱100℃ 10min殺死細菌，用無菌生理鹽水稀釋成6×108 細菌/ml備用；
2. 用血紅蛋白吸管吸取受試者耳垂或指血40μl，立即加入盛有20μl肝素（濃度為20U/ml）的潔凈凹玻片的凹孔內，輕輕攪動混勻， 再加上述葡萄球菌菌液20μl充分混勻。然后置入鋪有濕紗布的有蓋容器內（此容器先放37℃溫箱中預溫），在37℃溫箱中作用30min，其間每隔10min搖勻一次；
3. 作用完畢，取1小滴混合液置于潔凈無油污的載玻片一端，推成薄片。待干后，用甲醇固定4～5min，鹼性美藍液染2～3min，置油鏡下觀察。隨機計數100個中性粒細胞，分別記錄發生吞噬和未吞噬的白細胞數，對有吞噬作用的白細胞，應同時記錄所吞噬的細菌數；
結果分析
吞噬細胞%=即100個中性粒細胞中吞噬有細菌的細胞數；
吞噬指數=將100個中性粒細胞所吞噬的細菌總數除以100，得到每個白細胞吞噬細菌的平均數，即為吞噬指數。

CD8(T細胞)兔單克隆抗體

我司還提供其它進口或國產試劑盒：登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲病、違禁品濫用、肺炎球菌、軍團菌、化妝品檢測、食品安全檢測等試劑盒以及日本生研細菌分型診斷血清、德國SiFin診斷血清、丹麥SSI診斷血清等產品。

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【公司名稱】 廣州健侖生物科技有限公司
【市場部】     歐

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【騰訊  】 
【公司地址】 廣州清華科技園創新基地番禺石樓鎮創啟路63號二期2幢101-103室

2. Cytotoxicity test
Detection of CTL cytotoxicity can be used to detect NK cell killing activity, only the proportion of target cells is not the same, now LDH release method is briefly described as follows:
① target cells used as stimulating cells or allogenic EBV-LCL cells, adjusted to 1 × 105 / ml;
② effector cells induced by the above-mentioned specific CTL, transferred to 2.5 × 106 / ml;
(3) Take 0.1ml each in a 96-well plate (effect / target ratio of 25: 1); gently blow to mix the two; at the same time set the target cells to release the control group ) And maximal release control (0.1 ml target cells and 0.1 ml 1% NP-40);
④ After centrifugation at 1000rpm / min for 2min, incubate for 4hr at 37 ° C in a 5% CO2 incubator
⑤ enzymatic color reaction: see "NK cell killing experiment."
The function of polymorphonuclear leukocyte (PMN), including adhesion, migration, phagocytosis and so on, is an important part of the body's natural immunity.
Reagents and materials
Staphylococcus aureus
2. Broth culture medium
3 alkaline blue liquid
Method of operation
1. Bacterial liquid preparation Staphylococcus aureus inoculated in, put 37 ℃ incubator for about 12h; water bath heated to 100 ℃ 10min to kill bacteria, diluted with sterile saline into 6 × 108 bacteria / ml spare;
2. Hemoglobin pipette to absorb the subject's earlobe or finger blood 40μl, immediay added 20μl heparin (concentration of 20U / ml) clean concave glass concave wells, gently agitated and mix, coupled with the Staphylococcus aureus Mix well 20μl. Then placed in a covered container wet gauze (the container first pre-37 ° temperature incubator), 37 ° C incubator role in 30min, during which shake once every 10min;
3. The effect is completed, take a small drop of liquid mixture placed in clean oil-free one end of the slide, pushed into thin slices. To be dry, fixed with methanol 4 ~ 5min, alkaline methylene blue dye stained 2 ~ 3min, set the oil microscope observation. Randomly counting 100 neutrophils were recorded phagocytic and non-phagocytic leukocytes were phagocytosis of white blood cells, should also record the number of bacteria phagocytosed;
Result analysis
% Of phagocytes = number of cells engulfed by bacteria in 100 neutrophils;
Phagocytosis Index = Divide the total number of bacteria phagocytosed by 100 neutrophils by 100 to get the average number of phagocytic bacteria per leukocyte, which is the phagocytic index.