| 概述 |
|
| 別名 | Rabbit Anti-VEGF Polyclonal Antibody VEGF抗體;Vascuoar endothelial growth factor A; VEGF A; vascular endothelial growth factor A isoform 2 precursor; Vegf; VEGFA; MGC70609; MVCD1; VEGF; VPF; VEGFA_HUMAN; Vascular endothelial growth factor A; VEGF-A; Vascular permeability factor; VPF; VEGF A Precursor. |
| 宿主 | Rabbit |
| 特異性 | VEGF |
| 反應種屬 | Human; |
| 預測反應種屬 | Dog;Pig;Cow;Horse;Rabbit;Sheep; |
| 應用 | Rabbit Anti-VEGF Polyclonal Antibody WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復） |
| 免疫原 | KLH conjugated synthetic peptide derived from human VEGF:81-132/232 |
| 性能 |
|
| 濃度 | 1mg/ml |
| 純化方法 | affinity purified by Protein A |
| 類型 | Polyclonal Antibody |
| 同種型 | IgG |
| 儲存/保存方法 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 存儲溶液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 研究領域 | 腫瘤 心血管 細胞生物 免疫學 信號轉導 生長因子和激素 血管內皮細胞 |
| 靶標 |
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| 背景說明 | This gene product is a member of the PDGF/VEGF growth factor family. It is a mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis, endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Also, alternative translation initiation from non-AUG (CUG) and AUG start sites in some transcript variants, give rise to additional isoforms. [provided by RefSeq]. |
| 細胞定位 | 細胞外基質 分泌型蛋白
|
| UniProt | P15692 |
| 實驗結果圖 |
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| Tissue/cell: human brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min;
Incubation: Anti-VEGF Polyclonal Antibody, Unconjugated secondary primary antibody 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
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| 溫馨提示：本產品僅作科研實驗使用，不支持臨床等研究 |
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