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上海橋星貿易有限公司
主營產品： 進口透析袋,密理博ECL發光液,B27無血清培養基,Gibco膠原酶 |
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上海橋星貿易有限公司
主營產品： 進口透析袋,密理博ECL發光液,B27無血清培養基,Gibco膠原酶 |
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英文名稱：DMEM, Powder, High Glucose, Pyruvate
規格：10 x 1 L
性狀：干粉培養基
貨號：12800-017
12800-017DMEM高糖培養基描述
Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using themedia selector tool.
| With | Without |
| • High Glucose | • HEPES |
| • L-glutamine | • Sodium Bicarbonate |
| • Phenol Red | |
| • Sodium Pyruvate |
The complete formulation is available.
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing and Quality System
Gibco® DMEM is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards. For supply chain continuity, Life Technologies offers a comparable Gibco® DMEM product made in our Scotland facility (12800-116). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Powder forms of Gibco® cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).
12800-017DMEM高糖培養基規格
| Glutamine: | L-Glutamine |
|---|---|
| Phenol Red Indicator: | Phenol Red |
| Form: | Powder |
| Glucose: | High Glucose |
| Serum Supplementation: | Standard Serum Supplementation |
| HEPES Buffer: | No HEPES |
| Sodium Pyruvate Additive: | Sodium Pyruvate |
| Product Size: | 10 x 1 L |
| Sodium Bicarbonate Buffer: | No Sodium Bicarbonate |
| Shipping Condition: | Room Temperature |
12800 - DMEM, powder, high glucose, pyruvate
相關產品：
| 產品 | 規格 | * |
| DMEM高糖培養基Gibco12100-046 | 10x1L | 320 |
| RPMI1640培養基Gibco31800-022 | 10x1L | 290 |
| DMEM高糖培養基（含丙酮酸鈉）Gibco12800-017 | 10x1L | 300 |
| DMEM低糖培養基（含丙酮酸鈉）Gibco31600-034 | 10x1L | 300 |
| IMDM培養基Gibco12200-036 | 10x1L | 750 |
| M199培養基Gibco31100-035 | 10x1L | 360 |
| DMEM/F12培養基Gibco12400-024 | 10x1L | 500 |
| F12培養基Gibco21700-075 | 10x1L | 450 |
| MEM培養基Gibco41500-034 | 10x1L | 360 |
