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millipore轉印膜IPVH08130
Millipore 提供三種不同的Immobilon PVDF膜，是針對不同的蛋白質印跡應用的理想選擇。現在我們還提供采用按規格裁切的印跡方片膜和兩層過濾紙組成的印跡三明治。
與硝酸纖維素膜相比，Immobilon膜具有多種優點，它們不易破裂或卷曲，便于裁剪且在切割過程中不會碎裂。它們還有低背景，廣泛溶劑相容性以及優異的著色能力。此外，它們可以重復檢測。
Description Catalogue Number IPVH08130 Trade Name
Description Immobilon-P PVDF Membrane Background Information Overview
- Millipore offers four different Immobilon PVDF membranes, each optimized for different protein blotting applications.
Immobilon PVDF membranes provide many advantages compared to nitrocellulose. They won’t crack or curl, and they can be cut without fracturing. They also have low background, broad solvent compatibility, and superior staining capabilities. In addition, they can be reprobed multiple times.
Immobilon-P Transfer Membrane:
- 45 µm pore size Recommended for most western blotting, especially proteins greater than 20 kDa
- Millipore's Rapid Immunodetection Protocol reduces detection times by up to 2 hours by eliminating membrane blocking and several wash steps with no loss of sensitivity or specificity
Immobilon-E Membrane:
- the only PVDF membrane that wets out in aqueous buffers, eliminating the methanol pre-wet step.
- 45 µm pore size recommended for most western blotting, especially proteins greater than 20 kDa
Blotting Sandwiches:
- When you need to process multiple blots, blotting sandwiches are a convenient, time-saving choice. Our sandwiches include sheets of Immobilon-P membrane interleaved with pre-cut sheets of chromatography-grade blotting filter paper and are compatible with any standard wet transfer device.
Blotting Filter Paper:
- Chromatography-grade blotting filter paper pre-cut to the most popular western blotting sizes.
Immobilon-PSQ Membrane:
- 0.2 µm pore size and a large internal structure
- Higher protein adsorption and sequencing yields than other membranes
- Recommended for blotting proteins less than 20 kDa
- Prevents blow-through of low molecular weight proteins
Immobilon-FL Membrane:
- The first transfer membrane optimized for fluorescence applications
- Extremely low background improves sensitivity of all fluorescence detection protocols
- Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
- Ideal for multiplexing and chemifluorescence applications
Immobilon® PVDF membranes are offered in four types, each optimized for a different protein blotting application. Convenient blotting sandwiches feature pre-cut sheets of membrane and blotting filter paper. Immobilon® PVDF membranes have high protein adsorption, so you won’t lose proteins during transfer or reprobing. The open pore structure makes it easy to access bound proteins and remove unbound probes. In addition, Immobilon® PVDF membranes optimized for fluorescent blots dramatically increase signal-to-noise ratios for high sensitivity in quantitative, multiplexing applications.
Features & Benefits:
- Won’t crack, curl or fracture when cut
- Low background
- Superior staining capabilities
- Can be reprobed multiple times
Applications:
- Western Blotting, Immunoblotting, Dot Blotting, Protein Sequencing;
- Compatible with Chemiluminescent, Fluorescent, Radioactive, Chromogenic, and Chemifluorescent Detection
- Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
References
Product Information Detection method Chromogenic Radioactive Fluorescent Chemiluminescence Compatible Stains
Filter Code IPVH Filter Color White
Applications Application 10 sheets, 8.5 cm x 13.5 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting. Key Applications
Biological Information Primary Binding Mechanisms Electrostatic, hydrophobic BSA Adsorption 215 µg/cm2 Goat IgG Adsorption 294 µg/cm2 Insulin Adsorption 160 µg/cm2 Wettability Hydrophobic
Physicochemical Information Pore Size 0.45 µm
Dimensions Filter Surface Plain Filter Dimensions 8.5 cm x 13.5 cm
Materials Information Chemistry
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Packaging Information Material Size 10
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