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High-Resolution Separation of Proteins and Peptides
The Acclaim® 300 features 3 µm silica particles for rapid analysis of complex protein digests. The Acclaim 300’s stable bonding make these columns compatible with a wide variety of applications, including LC/MS methods.
Designed for high-resolution peptide mapping and protein separations
High-efficiency 3 µm spherical silica substrate
High-performance bonding chemistry on 300 Å pore silica
Application tested for suitablility in peptide mapping
Reproducible for dependable results
LC/MS compatible
Manufacture of these columns starts with ultrapure silica that contains minimal concentrations of metal contaminants, minimizing the tailing effects of residual, exposed silanol groups. Exhaustive bonding and end-capping techniques result in stable bonding and columns that exhibit predictable reversed-phase separations with minimal secondary interactions.
The use of a 3 µm silica particle reduces limits for diffusion of the analytes into the stationary phase, resulting in fast, high-resolution separations. Compared to 5 µm column packings, the smaller particles support increased flow rates and shallower gradients on shorter columns, for faster separations. Dionex tests each bonded silica lot for peptide selectivity to ensure reproducible performance.
| Acclaim PepMap for TFA-Free Peptide Mapping Specifications | |||
|---|---|---|---|
| Column type | Nano | Capillary | Micro |
| Column i.d. | 75 µm | 300 µm | 1.0 mm |
| Length | 50, 150, 250, and 500 mm | 50, 150, and 250 mm | 50, 150, and 250 mm |
| Recommended Flow Rate | 200 nL/min | 4 µL/min | 40 µL/min |
| Packing Material | C18, 100 Å, 3 µm | C18, 100 Å, 3 µm | C18, 100 Å, 3 µm |
| C18, 300 Å, 5 µm | C18, 300 Å, 5 µm | C18, 300 Å, 5 µm | |
| C8, 100 Å, 3 µm | C8, 100 Å, 3 µm | C8, 100 Å, 3 µm | |
| C8, 100 Å, 5 µm | C8, 100 Å, 5 µm | C8, 100 Å, 5 µm | |
| C4, 300 Å, 5 µm | C4, 300 Å, 5 µm | C4, 300 Å, 5 µm | |
| Column | Fused-silica | Glass Lined Tubing | Stainless steel |
| Column Inlet | 1/16-in. PEEK sleeve adapter (provided) nanoViper (optional) | 1/16-in. universal fitting nanoViper (optional) 50µm i.d. PEEK sheathed fused silica with nanoViper fittings | 1/16-in. PEEK tubing nanoViper (optional) 75µm i.d. PEEK sheathed fused silica with nanoViper fittings |
| Column Outlet | Fused-silica 25-µm i.d./ 280-µm o.d. | Fused-silica 75-µm i.d./ 280-µm o.d | 1/16-in. PEEK tubing nanoViper (optional) 75µm i.d. PEEK sheathed fused silica with nanoViper fittings |
| MS Outlet | 1-m Length | ||