Recombinant Cartilage Intermediate Layer Protein (CILP)

軟骨中間層蛋白(CILP)重組蛋白

[ PROPERTIES ]

Source:Prokaryotic expression

Host: E.coli

Residues: Ile603~Ala846

Tags:N-terminal His and GST Tag

Subcellular Location: Secreted

Purity: > 97%

Traits:Freeze-dried powder

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% skl, 5%Trehalose.

Original Concentration:50µg/mL

Applications:Positive Control; Immunogen; SDS-PAGE; WB.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point:6.1

Predicted Molecular Mass:  57.6kDa

Accurate Molecular Mass: 58kDa as determined by SDS-PAGE reducing conditions.

[ USAGE ]

Reconstitute in ddH₂O to a concentration of 0.1-0.2 mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

           Store at 2-8oC for one month.

           Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

[SEQUENCE ]

[  IDENTIFICATION  ]

[ IMPORTANT NOTE ]

The kit is designed for research use only, we will not be responsible for any issue if the kit was used in clinical diagnostic or any other procedures.

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