Recombinant Phospholipase C Beta 4 (PLCb4)

磷酯酶Cβ4(PLCb4)重組蛋白

[ PROPERTIES ]

Residues: Ala2~Gln250

Tags:Two N-terminal Tags, His-tag and T7-tag

Accession:Q15147

Host:E. coli

Purity: >95%

Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).

Formulation:Supplied as lyophilized form in 20mM Tris,150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 5.3

Predicted Molecular Mass: 32.1kDa

Applications:SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile ddH₂O..

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

           Store at 2-8oC for one month.

           Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

[SEQUENCE ]

The sequence of the target protein is listed below.

AKPYEFNWQ KEVPSFLQEG AVFDRYEEES FVFEPNCLFK VDEFGFFLTWRSEGKEGQVLECSLINSIRS GAIPKDPKIL AALEAVGKSE NDLEGRIVCV CSGTDLVNIS FTYMVAENPEVTKQWVEGLR SIIHNFRANN VSPMTCLKKH WMKLAFM NGKIPVRSIT RTFASGKTEKVIFQALKELG LPSGKNDEIE PTAFSYEKFY ELTQKICPRT DIEDLFKKIN GDKTDYLTVDQLVSFLNEHQ

[  IDENTIFICATION  ]

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