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Neuro Probe AA96, AB96, and AC96 chemotaxis chambers use framed filters on which adherent cells can be stained and counted under a microscope or counted in an automated fluorescence reader. The A-Series 96-well chemotaxis chambers consist of reusable precision-machined acrylic top and bottom plates, assembly hardware, and a silicone gasket. The bottom plate has 96 wells, and corresponding holes in the top plate form the 96 upper wells. The framed filter separates the upper and lower wells.
During incubation chemoattractants added to the lower wells act on cells in a suspension added to the upper wells. Cells migrate through the filter, and migrated adherent cells on the filter are counted.
The framed filter consists of a filter membrane bonded to a rigid frame that can be conveniently handled without forceps. The filter snaps accuray into the chamber, and after incubation the filter can be held by its frame while wiping, staining, and preparing to count cells. The external dimensions of the frame and the format of the 96 sites on the filter are designed to fit standard fluorescence readers, so fluorescent-tagged adherent cells can be automatically counted.
The top plate has six registration pins to ensure easy and accurate assembly. The silicone gasket is punched with holes corresponding to the registration pins and is installed on the top plate prior to assembly. The framed filter fits snugly against the pins. The gasket forms a seal when sandwiched with the filter between the top and bottom plates. Overall dimensions of the assembled chamber, excluding hardware, is 5.75″ x 5.00″ x .90″.
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