| I. Background | |
| HHLA2（也稱為 B7H5、B7H7 或 B7y）是 B7 家族的一員，它是 I 型跨膜蛋白，由具 有串聯 IgV1-IgC-IgV2 結構域的細胞外部分、跨膜區域和細胞質尾部組成。HHLA2 低表達 于胎盤、腸道上皮細胞等，高表達于多種實體瘤（如肺癌、乳腺癌、結直腸癌、腎癌）及 炎癥微環境。HHLA2 具有雙向免疫調節功能：結合抑制性受體 KIR3DL3（NK 細胞）和未 知 T 細胞受體，傳遞抑制信號，減弱抗腫瘤免疫應答；通過受體 TMIGD2 激活 PI3K-AKT 通路，促進 T 細胞存活及記憶形成。 | |
| II. Description | |
HHLA2(B7H7) aAPC Cell 作 為 KIR3DL3 Effector Reporter Cell 或 TMIGD2 Effector Reporter Cell 的 靶 細 胞 ， 很好的模擬了體內 KIR3DL3/ HHLA2(B7H7) 或TMIGD2/HHLA2(B7H7)的信號轉導過程，原理見下圖所示。  Figure 1. HHLA2(B7H7) aAPC Cell 細胞模型原理圖 | |
| II. Introduction | |
| Expressed gene: | HHLA2(B7H7) |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | F12K+10%FBS+2ug/ml puromycin+500ug/ml hygromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| III. Representative Data | |
Figure 1.Recombinant HHLA2(B7H7) aAPC Cell stably expressing HHLA2(B7H7). | |
Figure 2. Dose Response of HHLA2 Blocking Abs in KIR3DL3 Effector Reporter Cells (C1)With HHLA2(B7H7) aAPC Cell(C1). | |
Figure 3. Dose Response of HHLA2 Blocking Abs in TMIGD2 Effector Reporter Cells (C1) With HHLA2(B7H7) aAPC Cell. | |
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