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產品英文名稱：Protein A ELISA kit
產品中文名稱：蛋白質 A ELISA 試劑盒

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Protein A ELISA kit 的應用
本試劑盒是用于蛋白試劑的定量測定，只用于研究和制造，不用于人類或動物的診斷應用。該套件是一個修改后的F400試劑盒，廣大范圍的產品已通過法律的廣 泛的驗證。F400和F400Z套件使用相同的組件除了共軛的過氧化物酶標記。共軛用在F400Z試劑盒，F401Z是一種特殊提供不確定的樣本不屈服在 F400 KIT可接受的蛋白質A恢復的配方。具有該試劑盒回收增強試劑（ F404）必須重新結合F401Z共軛先驗分析。這是任可接受的精度和特異性的樣品類型的試劑盒的驗證zui終用戶的責任。

Protein A ELISA kit 的英文描述
This NEW PROTEIN A ELISA kit is designed to overcome low analytical recovery of Protein A encountered in many commercial kits. Most low recovery samples are either incomplete antibody fragments or so-called fusion proteins where the antibody structure is linked to another non-antibody molecule. The properties of such unnatural drug substance constructs, means that conventional sample treatment procedures are ineffective in dissociation, denaturation, precipitation, and removal of the drug substances prior to the Protein A detection assay. Any un-denatured drug substance remaining in the sample after the treatment step can reassociate with leached Protein A, resulting in under recovery by ELISA.
The #F400Z  Protein A ELISA kit has been qualified using several problematic samples and found to yield acceptable recovery on all. This kits uses the same antibodies, standards and sample treatment protocol as in our widely used #F400 kit. The only difference is that the #F400Z kit contains a "Recovery Enhancer" solution which must be combined with the HRP conjugated anti-Protein A antibody, #F401Z, prior to use. The "Recovery Enhancer" prevents the re-association of any drug substance that was not compley removed during the sample treatment step.
The #F400Z kit will accuray quantitate the unnatural construct of Protein A, MabSelect SuRe™ as well as other conserved, natural and recombinant Protein A constructs. Because not all drug substances will respond equally to sample treatment procedures, your lab must validate each new sample type and drug substance for acceptable analytical recovery before reporting results.

蛋白質 A ELISA 試劑盒的使用步驟規則
The Protein A assay is a two-site immunoenzymetric assay. Samples containing Protein A are first diluted with a sample denaturing buffer. This reagent dissociates the Protein A from the product antibody. Samples are then heated in a dry heating block or boiling water bath to denature and precipitate the product antibodies. After a centrifugation step to pellet the denatured product antibody, the samples are then reacted in microtiter strips coated with a polyclonal anti-Protein A capture antibody. A second anti-Protein A antibody labeled directly with Horse Radish Peroxidase (HRP) enzyme is simultaneously reacted forming a sandwich complex of solid phase antibody-Protein A-HRP labeled antibody. After a wash step to remove any unbound reactants, the strips are then reacted with tetramethyl benzidine (TMB) substrate. The amount of hydrolyzed substrate is read on a microtiter plate reader and will be directly proportional to the concentration of Protein A present. Accurate quantitation is achieved by comparing the signal of unknowns to Protein A standards assayed at the same time. 

Protein A ELISA kit 的保存和穩定性
* All reagents should be stored at 2C to 8C for stability until the expiration date printed.
*After prolonged storage, you may notice a salt precipitate and/or yellowing of the wash concentrate. These changes will not impact assay performance. To dissolve the precipitate, mix the wash concentrate thoroughly and dilute as directed in the ‘Preparation of Reagents’ section. Reconstituted wash solution is stable until the expiration date of the kit.

蛋白質 A ELISA 試劑盒的使用注意事項
*只用于研究或制造。
*停止反應試劑是0.5M H2SO4。避免接觸眼睛，皮膚，和服裝。在用本試劑盒的試劑濃度，其他沒有被認為是有害的。
*本試劑盒只能由合格的技術人員使用。

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