NUCLEOSIL® MPN

Silica-based HPLC phases for RP chromatography
of biological macromolecules

Features

Silica-based reversed phase materials with monomerically bonded alkyl chains, brush type structure
predominantly hydrophobic forces with a small portion of hydrophilic interactions

NUCLEOSIL® 100-5 C18 MPN: octadecyl phase, particle size 5 µm, pore size 100 Å ? USP L1
Dynamic protein binding capacity per g packing: 6 mg BSA, 110 mg cytochrome C

NUCLEOSIL® 300-5 C4 MPN: butyl phase, particle size 5 µm, pore size 300 Å ? USP L26
Dynamic protein binding capacity per g packing: 14 mg BSA, 27 mg cytochrome C
especially suited for the purification of larger, hydrophobic peptides and very different proteins
For more applications click here.

pH working range 2–8, max. working pressure 250 bar
Maximum separation efficiency can be achieved when the injected protein mass does not exceed 1–2 %of the maximum protein loading capacity.
Eluent in column methanol

Ordering information

Length 250 mm

CC guard columns*

EC analytical columns   

NUCLEOSIL® 100-5 C18 MPN

4 mm ID

720231.40

NUCLEOSIL® 300-5 C4 MPN

4 mm ID

720245.40

721113.40

* CC 8/4 guard column cartridges are used for EC columns of 4 mm ID with guard column adapter EC (REF 721359). Guard column cartridges in packs of 2, EC columns in packs of 1