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當前位置：> 供求商機> Glucose-6-phosphate Dehydrogenase葡萄糖-6-磷酸脫氫酶（6-磷酸葡萄糖脫氫酶）

Glucose-6-phosphate Dehydrogenase葡萄糖-6-磷酸脫氫酶（6-磷酸葡萄糖脫氫酶）
Glucose-6-phosphate Dehydrogenase葡萄糖-6-磷酸脫氫酶（6-磷酸葡萄糖脫氫酶）

所在地：Sigma

貨物所在地：上海上海市

更新時間：2025/3/12 21:00:48

訪問次數：266

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初級會員·13年
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曹女士
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86-21-34615995
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供應簡介
Enzyme Commission （EC） Number: 1.1.1.49
CAS Number: 9001-40-5
Molecular Weight:
128 kDa （gel filtration）9
103.7 kDa for the dimer （equilibrium sedimentation）1
Approximay 50 kDa for the monomer


詳細介紹

 

G-6-P-DH; Glucose-6-phosphate Dehydrogenase;葡萄糖-6-磷酸脫氫酶;6-磷酸葡萄糖脫氫酶
Product Description
CAS Number: 9001-40-5
Molecular Weight:
128 kDa （gel filtration）9
103.7 kDa for the dimer （equilibrium sedimentation）1
Approximay 50 kDa for the monomer （denaturing gel electrophoresis）2
ëmax: 280.5 nm1
Extinction Coefficient: E0.1% = 1.15 （280.5 nm, 0.09 M
Tris-HCl, pH 7.2）1
Glucose-6-Phophate Dehydrogenase （G-6-PDH） consists of two subunits of equivalent molecular weight.The amino acid sequence of the monomer has been published
G-6-PDH has been utilized in assays for nicotinamideadenine dinucleotide and tissue pyridine nucleotides
The KM value for NAD+ as a substrate is approximay 1.8 times better than that for NADP+. At pH 7. in Tris buffer, KM = 5.3 x 10-4 M for glucose-6-phosphate and KM = 0.99 x 10-4 M for NADP+.6 Binding constants have been reported for the native and pyridoxalmodified
enzyme. A lysine is in the active site （modified by pyridoxal phosphate）.
G-6-PDH can be reactivated from urea-denatured solutions.
Glucose 6-phosphate dehydrogenase is a key regulatory enzyme in the first step of the pentose phosphate pathway. G-6-P-DH oxidizes glucose-6-phosphate in the presence of NADP+ to yield 6-phosphogluconate. Polyacrylamide gel electrophoresis, activity staining, and anti-yeast G-6-PDH antibody immunoblotting studies have indicated that G-6-PDH is a glycoprotein.
    This product is approximay 25% protein; the remainder is the buffer from which it is lyophilized. The product is lyophilized in the presence of a neutral, colloidal stabilizer, Tris buffer, and a trace of magnesium chloride. The stabilizer added to this product is an inert neutral polymer, which is not expected to interfere in a biological assay. It protects the product through the lyophilization process. A DEAE ion exchange column can be used with a buffer
of approximay pH 7.3 to remove this stabilizer. Product No. G 5760 is an ammonium sulfate
suspension of the enzyme and does not contain this stabilizer.
Precautions and Disclaimer
For Laboratory Use Only. Not for drug, household or other uses.
Preparation Instructions
The product can be dissolved at 1 mg/ml in 5 mM glycine buffer, pH 8, （with or without 0.1% BSA） or in deionized water at 1 mg/ml （buffer is preferable）.
Storage/Stability
Solutions dissolved at 1 mg/ml in 5 mM glycine buffer, pH 8, （with or without 0.1% BSA） or in deionized water （1 mg/ml） can be aliquoted and stored frozen for approximay 2 months. It is best to subject the aliquot to no more than one freeze/thaw cycle. For storage in the refrigerator, use an ammonium sulfate suspension of this enzyme （Product No. G 5760）.
Ordering Information

貨號
名稱
產地
規格
報價
*（元）
庫存
G5885
G6PD 葡萄糖-6-磷酸脫氫酶
1000U
2063
980
是
G5885
G6PD 葡萄糖-6-磷酸脫氫酶
Sigma
2000U
2995
1764
是
G5885
G6PD 葡萄糖-6-磷酸脫氫酶
Sigma
10KU
10325
6195
是

 

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