24-square deepwell microplate 225 rpm

24-square deepwell microplate 225 rpm

4平方深井微板孔內流體力學

對于24個方形深孔微量滴定板，通常使用2.5-3.5毫升的培養體積**，在搖動器上轉速為300毫米（左側為照片和視頻）或在50毫米振幅的振動篩上（在右邊的照片和視頻上為225轉/分鐘）。在這兩種條件下，2.5毫升培養物的氧傳輸速率約為35-39毫摩爾O2 L-1 H-1（對應于180～200 H-1的K La值）。*優選使用培養體積。2.5毫升，特別是當三明治蓋必須偶爾取下時（例如用于取樣或喂食）

50毫米振動篩振幅•225轉/分

Hydrodynamics inside wells of a 24-square deepwell microplate
For 24-square deepwell microtiter plates, culture volumes between 2.5 ml and 3.5 ml are generally used**, either at 300 rpm on a shaker with a 25 mm amplitude (photo and video on the left) or at 225 rpm on a shaker with a 50 mm amplitude (photo and video on the right).Under both conditions oxygen transfer rates for 2.5 ml cultures are approximately 35-39 mmol O2 l-1 h-1  (corresponding to k La values of 180-200 h-1).** preferably use a culture volume of 2.5 ml, especially when the sandwich cover has to be take off occasionally (e.g. for sampling or feeding)
50 mm shaker amplitude•225 rpm

 注：楊清辰發布