8-Well Cell Settling Chamber該腔室由兩個(gè)精密加工的丙烯酸板，一個(gè)硅酮墊圈，和組裝硬件。較低的平板有凹槽，可容納25 x 75毫米的玻璃顯微鏡載玻片。上片有8個(gè)通孔，上片夾在下片顯微鏡載玻片上形成280l孔。墊圈和一張預(yù)沖濾紙(股票號(hào)DC8WF1)夾在兩個(gè)板之間，導(dǎo)致細(xì)胞懸浮液的液體吸走，因?yàn)榧?xì)胞落在幻燈片上，留下細(xì)胞的位置干燥。為了獲得好的結(jié)果，可以在8個(gè)沉降點(diǎn)(編號(hào)DC8SC)周圍使用增強(qiáng)結(jié)合性能的顯微鏡載玻片和疏水性掩膜。這些幻燈片改善了細(xì)胞粘附性，也限制了管道解決方案的個(gè)別地點(diǎn)。然后可以在沒(méi)有串?dāng)_的情況下對(duì)固定的單元應(yīng)用不同的解決方案。在處理淋巴細(xì)胞等密度較低的細(xì)胞時(shí)，應(yīng)在裝有微板載體的離心機(jī)中旋轉(zhuǎn)腔體。離心機(jī)附件包(股票編號(hào)DC8AP)提供了一個(gè)適配器和配重用于此目的。每個(gè)腔室都附帶一個(gè)DC8協(xié)議供其使用和護(hù)理。滑槽尺寸:DC8 32mm2 280 L 25 x 75 x 1 mm

8-Well Cell Settling Chamber

The chamber consists of two precision-machined acrylic plates, a silicone gasket, and assembly hardware. The lower plate has a recess to fit a 25 x 75mm glass microscope slide. The upper plate has eight through holes, which form 280µL wells when the upper plate is clamped onto the microscope slide in the lower plate. The gasket and a sheet of prepunched filter paper (Stock # DC8WF1) pinched between the plates cause liquid in the cell suspension to wick away as the cells settle onto the slide, leaving the cell sites dry.

For best results microscope slides with enhanced binding properties and a hydrophobic mask around the eight settling sites (Stock # DC8SC) are available. These slides improve cell adherence and also restrict pipetted solutions to individual sites. Different solutions can then be applied to settled cells without crosstalk.

When working with cells of relatively low density, such as lymphocytes, the chamber should be spun in a centrifuge equipped with a microplate carrier. A centrifuge accessory pack (Stock # DC8AP) provides an adapter and counterweight for this purpose.

Accompanying each chamber is a DC8 protocol for its use and care.