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進口SE型極化熒光光度計
High Sensitivity - Picomolar
Quantitative results
Minimal sample volumes and preparation
Rapid measurments
Programable Data collection
Variable Temperature Sample chamber
Easy to change external filter holders
Standard System 360-700nm
Expanded System 250-850nm
The Fluorescence Polarization Systems provide a rapid and precise way to measure equilibrium binding and/or molecular degradation, with picomolar sensitivity, for a wide range of biological molecules. Fluorescence polarization takes advantage of the change in the speed of rotation that occurs when a fluorescent molecule binds to another molecule. The Systems are useful in performing immunoassays, determining antibody specificity and affinity, and providing protein-DNA and receptor-ligand binding measurements. Affinity binding is measured directly in solution, with each data point obtained in just a few seconds (6 seconds minimum). This simple, direct approach eliminates the need for any filtration, gel mobility shift assays, or immobilization techniques, with fluorescent labeling eliminating the use of radioisotopes
The Fluorescence Polarization Systems are stand alone, bench-top instruments that offer sensitive polarization measurement (requiring as little as 10fmol/mL of fluorescently labled sample), in a single-tube-format. The instruments use standard disposable glass cuvettes and their small footprint
maximizes precious bench space. The instruments have an onboard operating software that lets the user store up to 99 user-defined protocols and send data directly to a printer or your personal computer for analysis.
Fluorescence Polarization System features:
Expanded System - 250-850nm combination Quartz Halogen and Mercury Vapor Lamps
Polarizers - Glan-Taylor prisms with Extinction coefficient >10,000 from 280-900nm
Standard System - 360-700nm Quartz Halogen lamp
Polarizers - Film type with Extinction coefficient >10,000 from 400-700nm
Both Instruments feature:
Filters - Special fluorescein Interference filters (490nm Excit. and 525nm Emiss.)
Easy filter exchange for use with wide range of fluophores
Sample format - Single tube, Cell holders for 6mm (100µl) and 10mm (500µl)
(5mm (25µl) is optional
Precision - <2mP S.D. at 1nM fluorescein for 0.5mL sample
Sensitivity - Accurate to 10-11M fluorescein in polarization mode
and 10-13M fluorescein in intensity mode
Temperature Control - Variable from 5°C to 75°C in 1°C increments,±1°C
Read Time - Minimum 6 seconds, variable number of read cycles
Programmable Protocols - 99 user defined, password protected
Interfaces - RS232-C, DB25 parallel
Printer - External Thermal (parallel) 40 column
LxH - 20.5" x 14.75" x 9.25" (52.6x37.8x23.7cm)
Net Weight - 35lbs. (15.9kg)
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