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供貨周期	一周	應用領域	醫療衛生,化工,生物產業,制藥
主要用途	科學研究

NCI-H508 人結腸直腸腺癌

ATCC^® Number:

CCL-253™

Price:

Designations:	NCI-H508 [H508]
Depositors:	AF Gazdar
Biosafety Level:	1
Shipped:	frozen
Medium & Serum:	See Propagation
Growth Properties:	floating aggregates of round cells with some attached cells
Organism:	Homo sapiens （human）
Morphology:	epithelial
Source:	Organ: cecum Disease: colorectal adenocarcinoma
Cellular Products:	carcinoembryonic antigen （CEA）, 1709 ng/ml per 10 exp6 cells per 10 days
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
NCI-H508 人結腸直腸腺癌
Tumorigenic:	Yes
Antigen Expression:	Blood type A; Rh+; CA19-9 antigen
DNA Profile （STR）:	Amelogenin: X CSF1PO: 11,12 D13S317: 8,12 D16S539: 12 D5S818: 12 D7S820: 8,12 THO1: 9,9.3 TPOX: 8,11 vWA: 15,16
Cytogenetic Analysis:	modal number = 102; range = 71 to 131; most cells have DM's
Isoenzymes:	AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 1 PGM1, 1 PGM3, 1
Age:	55 years
Gender:	male
Ethnicity:	Caucasian
Comments:	This line was derived from a metastasis to the abdominal wall obtained from a patient after treatment with 5-fluorouracil. The cells contain Dopa decarboxylase, CA19-9 antigen and CEA but do not express the TAG-72 antigen.
Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing:	S
Preservation:	Culture medium, 95%; DMSO, 5%
References:	23066: Park JG, et al. Characteristics of cell lines established from human colorectal carcinoma. Cancer Res. 47: 6710-6718, 1987. PubMed: 3479249

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