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pCMV-VSV-G載體序列
最近更新時間：2013-8-2
提 供 商：上海起發實驗試劑有限公司資料大小：6.4KB
文件類型：未知文件下載次數：700次
資料類型：瀏覽次數：4217次
pCMV-VSV-G載體序列說明書
| 型號 | 載體名稱 | 出品公司 | 載體用途 |
| VMA0369 | pCMV-VSV-G | Addgene | 慢病毒載體 |
5' sequencing primer: T7
Vector for constitutive expression of VSV-G glycoprotein. Also known as pHCMV-G or pHCMV-VSV-G.
Retroviruses are efficient tools for delivering heritable genes into the genome of dividing cells. pCMV-VSV-G expresses the G glycoprotein of the vesicular stomatitis virus （VSV-G） under the control of the CMV immediate-early promoter. VSV-G is used in pseudotyping of Moloney Murine Leukenia Virus（MMLV）-based retroviral vectors by mediating viral entry. VSV-G interacts with phospholipidcomponents of the target cell membrane and fosters the fusion of viral and cellular membranes. VSV-G does not require a cell surface receptor and can serve as a surrogate viral envelope protein.
The vector contains the ampicillin-resistance gene for propagation and antibiotic selection in bacteria.
