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RNAi-Ready pSIREN-RetroQ載體說明書
最近更新時間：2013-7-24
提 供 商：上海起發實驗試劑有限公司資料大小：90.3KB
文件類型：PDF 文件下載次數：332次
資料類型：瀏覽次數：2775次
RNAi載體，RNAi-Ready pSIREN-RetroQ載體，Clontech
RNAi-Ready pSIREN-RetroQ
| 型號 | 載體名稱 | 出品公司 | 載體用途 |
| VRC0345 | RNAi-Ready pSIREN-Retro Q | Clontech | RNAi載體 |
RNAi-Ready pSIREN-RetroQ is a self-inactivating retroviral expression vector designed to express a small hairpin RNA （shRNA） using the human U6 promoter （PU6; RNA Pol III-dependent）. RNAi-Ready pSIREN-RetroQ is provided as a linearized vector digested with BamH I and EcoR I. It is used for targeted gene silencing when an oligonuceotide encoding an appropriate shRNA is ligated into the vector. You can transfect your pSIREN-RetroQ construct as a plasmid expression vector, or—upon transfection into a packaging cell line—this vector can transiently express, or integrate and stably express a viral genomic transcript containing the human U6 promoter and the shRNA. The vector contains a puromycin resistance gene for the selection of stable transfectants.
