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pRevTRE說明書
最近更新時(shí)間：2013-7-23
提 供 商：上海起發(fā)實(shí)驗(yàn)試劑有限公司資料大小：245.5KB
文件類型：PDF 文件下載次數(shù)：271次
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Clontech 四環(huán)素調(diào)控系統(tǒng) pRevTRE載體
pRevTRE
| 型號(hào) | 載體名稱 | 出品公司 | 載體用途 |
| VSC0468 | pRevTRE | Clontech | 四環(huán)素調(diào)控系統(tǒng) |
Promotor: LTR
Size: 6500
Selectable Marker: Hygromycin
Constitutive: Inducible
Viral/Non-Viral: Retroviral
pRevTRE is a retroviral Tet response vector that expresses a gene of interest from the Tet-response element （TRE）. This vector is derived from pLNCX, a retroviral vector created using elements of Moloney murine leukemia virus （MoMuLV） and Moloney murine sarcoma virus （MoMuSV） as described. The TRE contains seven direct repeats of the tetO operator sequence, upstream of a minimal CMV promoter, which can be bound by the tTA and rtTA transactivators. The 5' viral LTR controls expression of the transcript that contains Y+ （the extended viral packaging signal） and the hygromycin resistance （Hygr） gene for antibiotic selection in mammalian cells. The TRE is derived from vectors described previously. pRevTRE also includes the E. coli Ampr gene for antibiotic selection in bacteria.
The complete RevTet-Off and RevTet-On Systems also include the control vector pRevTRE-Luc, which was constructed by cloning the firefly luciferase gene into the HindIII/Cla I sites in the MCS of pRevTRE.
