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Matchmaker™ Library Construction & Screening Kits （pGBKT7，pGADT7，pGBKT7-53，pGADT7-T，pGBKT7-Lam）
最近更新時(shí)間：2013-7-23
提 供 商：上海起發(fā)實(shí)驗(yàn)試劑有限公司資料大小：964.4KB
文件類型：PDF 文件下載次數(shù)：498次
資料類型：瀏覽次數(shù)：6495次
pGBKT7-53
Bacteria Resistance：KanamycinMammalian Selection：-
載體特點(diǎn)：與pGADT7-T構(gòu)成Matchmaker Two-Hybrid System 3的雙雜交正對(duì)照。
pGBKT7-53 encodes a fusion of the murine p53 protein （a.a. 72–390） and the GAL4 DNA-BD （a.a. 1–147）. The murine p53 cDNA （GenBank Accession #K01700） was cloned into pGBKT7 at the EcoR I and BamH I sites. The p53 insert was derived from the plasmid described in Iwabuchi et al. （1993）; plasmid modification was performed at Clontech. pGBKT7-53 has not been sequenced.
pGBKT7-Lam
Bacteria Resistance：KanamycinMammalian Selection：-
載體特點(diǎn)：Matchmaker Two-Hybrid System 3的負(fù)對(duì)照。
pGBKT7-Lam is a negative control plasmid that encodes a fusionof the human lamin C protein （a.a. 66–230） and the GAL4 DNA-BD （a.a. 1–147）. The lamin C cDNA insert （GenBank Accession #M13451） was derived from the plasmid referenced in Bar et al. （1993a）. Plasmid modification was performed atBD Biosciences Clontech. Yeast cotransformed with pGBKT7-Lam and pGADT7-RecT, provide a measure of the backgroundthat is due to false-positive two-hybrid interactions. pGBKT7-Lam has not been sequenced.
