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CYT-436

重組人心肌營養素-1（CT-1）

2μg/10μg/1mg

Prospec

Introduction:
	Cardiotrophin-1 （CT-1） is a 201 amino acid member of the interleukin-6 superfamily. It was identified by its ability to induce hypertrophic response in cardiac myocytes. CT-1 mRNA levels were found both in cardiac myocytes and in cardiac nonmyocytes. CT-1 was also detected in abundance in normal adult human lung and was expressed in both fetal and adult airway smooth muscle cells. CT-1activates gp130 dependent signaling and stimulates the Janus kinase/signal transducers and activators of transcription （JAK/STAT） pathway to transduce hypertrophic and cytoprotective signals in cardiac myocytes. CT-1 has also a neurotrophic function. CT-1 deficiency causes increased motoneuron cell death in spinal cord and brainstem nuclei of mice during a period between embryonic day 14 and the first postnatal week. Moreover, CT-1 is a hepatocyte survival factor that efficiently reduces hepatocellular damage in animal models of acute liver injury. CT-1 expression is augmented after hypoxic stimulation and it can protect cardiac cells when added either prior to simulated ischaemia or at the time of reoxygenation following simulated ischaemia. CT-1 can induce expression of the protective heat shock proteins （hsps） in cardiac cells. Cardiotrophin-1 increased ventricular expression of ANP, brain natriuretic peptide （BNP） and angiotensinogen mRNA. CT-1 levels were significantly elevated in patients with heart failure, patients with dilatative cardiomyopathy, moderate/severe mitral regurgitation, stable and unstable angina and after acute myocardial infarction.
	Description:
	The Human Cardiotrophin His-Tagged Fusion Protein, produced in E. coli, is 22.5 kDa protein containing 200 amino acid residues of the human Cardiotrophin and 12 additional amino acid residues .
	Amino acid sequence:
	MRGSHHHHHH GSSRREGSLE DPQTDSSVSL LPHLEAKIRQ THSLAHLLTK YAEQLLQEYV QLQGDPFGLPSFSPPRLPVA GLSAPAPSHA GLPVHERLRL DAAALAALPP LLDAVCRRQA ELNPRAPRLL RRLEDAARQA RALGAAVEAL LAALGAANRG PRAEPPAATA SAASATGVFP AKVLGLRVCG LYREWLSRTE GDLGQLLPGG SA.
	Specificity:
	The amino acid sequence of the recombinant human cardiotrophin is 100% homologous to the amino acid sequence of the human cardiotrophin.
	Purification Method:
	Three-step procedure using affinity Ni-NTA chromatography and size exclusion chromatography before and after refolding.
	Purity:
	Purity of recombinant human cardiotrophin is >90% （SDS-PAGE analyzed）.
	Formulation:
	Sterile filtered and lyophilized from 0.5 mg/ml in 0.05M Acetate buffer pH4
	Reconstitution:
	Add 0.2ml of 0.1M Acetate buffer pH4 and let the lyophilized pellet dissolve compley. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10μg/ml. In higher concentrations the solubility of this antigen is limited.
	Storage:
	Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. Please avoid freeze-thaw cycles.
	Stability/Shelf Life:
	The lyophilized protein remains stable until the expiry date when stored at -20°C.
	Applications:
	Western blotting
	References:
	• Freed DH, Moon MC, Borowiec AM, Jones SC, Zahradka P, Dixon IM. CT-1 expression in experimental myocardial infarction and potential role in post-MI wound healing. Mol Cell Biochem. 2003 Dec; 254（1-2）: 247-56. • Freed DH, Borowiec AM, Angelovska T, Dixon IM. Induction of protein synthesis in cardiac fibroblasts by cardiotrophin-1: integration of multiple signaling pathways. Cardiovasc Res. 2003 Nov 1; 60（2）: 365-75. • Bristow MR, Long CS. Cardiotrophin-1 in heart failure. Circulation. 2002 Sep 17; 106（12）: 1430-2. • Liao Z, Brar BK, Cai Q, Stephanou A, O'Leary RM, Pennica D, Yellon DM, Latchman DS. Cardiotrophin-1 （CT-1） can protect the adult heart from injury when added both prior to ischaemia and at reperfusion. Cardiovasc Res. 2002 Mar; 53（4）: 902-10.
	Usage:
	Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.