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Sulfo-LC-SPDP, sulfosuccinimidyl 6-（3'-（2 pyridyldithio） pro介紹

Thermo Scientific BCA蛋白定量產(chǎn)品可以用于研究蛋白：蛋白間相互作用，衡量親和層析后的組分含量，估算從細胞裂解液里提取的膜蛋白含量和高通量篩選融合蛋白。

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Sulfo-LC-SPDP, sulfosuccinimidyl 6-（3'-（2 pyridyldithio） pro品牌介紹

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Sulfo-LC-SPDP, sulfosuccinimidyl 6-（3'-（2 pyridyldithio） pro

僅用于科研

Thermo Scientific Pierce Sulfo-LC-SPDP is a water-soluble, long-chain crosslinker for amine-to-sulfhydryl conjugation via NHS-ester and pyridyldithiol reactive groups that form cleavable （reducible） disulfide bonds with cysteines.

Sulfo-LC-SPDP is Sulfosuccinimidyl 6-（3'-[2-pyridyldithio]-propionamido）hexanoate, a heterobifunctional, thiol-cleavable and membrane permeable crosslinker. It contains an amine-reactive N-hydroxysuccinimide （NHS） ester that will react with lysine residues to form a stable amide bond. The other end of the spacer arm is terminated in the pyridyl disulfide group that will react with sulfhydryls to form a reversible disulfide bond.

Highlights:

• Releases a detectable by-product when reacted with a free sulfhydryl group; by measuring the release of pyridine-2-thione at 343 nm, the reaction can be easily followed
• Water soluble, amine reactive Sulfo-NHS ester reacts rapidly with any primary amine-containing molecule
• Disulfide bond in the spacer arm is readily cleaved by 10-50 mM DTT or TCEP at pH 8.5
• Spacer arm is also cleaved with 5% β-mercaptoethanol in SDS-PAGE sample loading buffer at 100°C for 5 minutes
• Cleavable crosslinker allows separation of crosslinked products

   

   

• Sulfo-LC-SPDP crosslinker is not membrane permeable, so crosslinking can be carried out on the cell surface