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Antigen
Ovalbumin Ab

SpeciesReactivity
Mouse

Form
Stds=0-400 ng/ml ; Sample=100 ul (1:100); Sensitivity= 5 ng/ml; 105 min assay; Quantitative, Ag-based Ab Assay, 96 tests

Samples
Serum. Plasma, Culture medium and other fluids may be adapted

KitContents
1. Ovalbumin coated strip plate (8 wells x 12 strips) 600-100-12. Mouse anti-Ovalbumin Ig?s Std A, (0 ng/ml), 0.65 ml 600-100-A3. Mouse anti-Ovalbumin Ig?s Std B, (25 ng/ml), 0.65 ml 600-100-B4. Mouse anti-Ovalbumin Ig?s Std C, (50 ng/ml), 0.65 ml 600-100-C5. Mouse anti-Ovalbumin Ig?s Std D, (100 ng/ml), 0.65 ml 600-100-D6. Mouse anti-Ovalbumin Ig?s Std E, (200 ng/ml), 0.65 ml 600-100-E7. Mouse anti-Ovalbumin Ig?s Std F, (400 ng/ml), 0.65 ml 600-100-F8. Antibody-HRP diluent, 12 ml 600-100-29. Anti-Mouse IgG-HRP Conjugate (100x),0.12 ml 600-100-310. Sample Diluent (10X), 10 ml SD-1011. Wash Buffer (100X), 10 ml WB-10012. TMB Substrate, 12 ml 8009113. Stop solution, 12 ml 8010114. Instruction Manual M600-100-OGG

SpCrossreactivity
ADI has developed a novel quantitative ELISA kit to measure anti-ovalbumin antibody types (IgGs, IgA, IgM, IgE) and their serum concentrations in mice exposed to ovalbumin. These kits will make it possible to compare the effects of various experimental manipulations on the production of anti-ovalbumin antibody type and its concentration. This kit measure total anti-ovalbumin IgG as the antibody conjugate used in the kit is not specific for any given specific isotype. We have other isotype specific anti-ovalbumin kit available (see below). The utility of the kit in other species (rat, human etc) have not been established.

MSDS
TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates

For research use only, not for diagnostic or therapeutic use.