活性重組蛋白IGF1 Protein
參考價(jià) | ¥500.00-¥9800.00 |
具體成交價(jià)以合同協(xié)議為準(zhǔn)
- 公司名稱 武漢瑾萱生物科技有限公司
- 品牌其他品牌
- 型號(hào)
- 所在地武漢市
- 廠商性質(zhì)生產(chǎn)廠家
- 更新時(shí)間2023/12/7 10:11:13
- 訪問(wèn)次數(shù) 31
規(guī)格
10ug | 500.00元 | 88888 件可售 |
20ug | 980.00元 | 88888 件可售 |
50ug | 1980.00元 | 88888 件可售 |
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供貨周期 | 現(xiàn)貨 | 應(yīng)用領(lǐng)域 | 醫(yī)療衛(wèi)生,化工,生物產(chǎn)業(yè),制藥,綜合 |
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種屬
Human
表達(dá)宿主
Pichia
描述
Recombinant Human IGF-I Protein is produced by Pichia expression system. The target protein is expressed with sequence (Gly49-Ala118) of human IGF-I (Accession #NP_001104755.1) fused with no additional amino acid.
標(biāo)簽
No tag
純度
> 95% by SDS-PAGE.
內(nèi)毒素
< 0.1 EU/μg of the protein by LAL method.
生物活性
Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED50 for this effect is typically 53.5-214.2 ng/mL.
制劑
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
儲(chǔ)存
Store the lyophilized protein at -20°C to -80°C for long term.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.未開(kāi)蓋的干粉蛋白在 -20°C至-80°C可保存12個(gè)月;
復(fù)溶之后,蛋白溶液在-20°C及以下可保存3個(gè)月,在2-8℃可保存1周。
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.未開(kāi)蓋的干粉蛋白在 -20°C至-80°C可保存12個(gè)月;
復(fù)溶之后,蛋白溶液在-20°C及以下可保存3個(gè)月,在2-8℃可保存1周。
復(fù)溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重組蛋白產(chǎn)品之后請(qǐng)檢查蛋白凍干粉末是否貼于瓶底,如果粉末浮起,開(kāi)蓋之前請(qǐng)先低溫離心。將蛋白用說(shuō)明書中的緩沖液復(fù)溶至0.1-0.5 mg/mL(請(qǐng)注意蛋白復(fù)溶濃度不能低于0.1 mg/mL),室溫平衡5-10 min保證充分溶解,復(fù)溶過(guò)程中請(qǐng)不要?jiǎng)×覝u旋及吹打蛋白溶液。如需長(zhǎng)期儲(chǔ)存,建議復(fù)溶時(shí)添加載體蛋白或者穩(wěn)定劑(如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖),同時(shí)將復(fù)溶后的蛋白溶液按照需求進(jìn)行分裝,儲(chǔ)存于-20°C至-80°C,隨取隨用,避免反復(fù)凍融。
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背景信息
基因ID
3479
Swiss Prot
P05019-1
別名
IGF-1;IGF-I;IGF1A;IGFI;IGF?I;MGF;IGF1