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化工儀器網>產品展廳>試劑標物>行業專用試劑>生化與分子生物學用試劑>abs04039 Siglec-3/CD33 (C-6His)

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abs04039 Siglec-3/CD33 (C-6His)

參考價 840
訂貨量 ≥1
具體成交價以合同協議為準

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供貨周期 一周 規格 10ug
概述
描述Recombinant Mouse Sialic Acid Binding Ig-like Lectin 3 is produced by our Mammalian expression system and the target gene encoding Asp18-Glu240 is expressed with a 6His tag at the C-terminus.
別名CD33/Myeloid cell surface antigen CD33/Sialic acid-binding Ig-like lectin 3/Siglec-3/Siglec3
形態Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4.
來源Human Cells
氨基酸序列DLEFQLVAPESVTVEEGLCVHVPCSVFYPSIKLTLGPVTGSWLRKGVSLHEDSPVATSDPRQLVQ KATQGRFQLLGDPQKHDCSLFIRDAQKNDTGMYFFRVVREPFVRYSYKKSQLSLHVTSLSRTPDI IIPGTLEAGYPSNLTCSVPWACEQGTPPTFSWMSTALTSLSSRTTDSSVLTFTPQPQDHGTKLTC LVTFSGAGVTVERTIQLNVTRKSGQMREVDHHHHHH
內毒素水平Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
Accession #Q63994
性能
背景Mouse myeloid cell surface antigen CD33(CD33) is a member of the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. CD33 contains one Ig-like C2-type domain and one Ig-like V-type domain. CD33 is a putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. CD33 preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, CD33 may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. CD33 induces apoptosis in acute myeloid leukemia. CD33 is becoming increasingly important as a target of antibody-mediated therapy in acute myeloid leukaemia (AML).
溶解方法Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
保存方法Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
純度Greater than 95% as determined by reducing SDS-PAGE.


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