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細(xì)胞培養(yǎng)invitrogen/Gibco胎牛血清(貨號16000-044)說明書

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gibco胎牛血清

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上海恪敏生物科技有限公司是一家集經(jīng)銷批發(fā)、招商代理的有限責(zé)任公司

生化部主營:生化試劑、抗體、蛋白/多肽、分子生物學(xué)、細(xì)胞生物學(xué)、Elisa試劑盒、發(fā)光試劑盒、培養(yǎng)基、細(xì)胞、蛇毒試劑、標(biāo)準(zhǔn)品、 標(biāo)準(zhǔn)溶液……(主營)試劑、正規(guī)品牌Elisa kit、抗體蛋白、分子生物、標(biāo)準(zhǔn)品(溶液)……
abcam、BD、Beckman、GE、invitrogen、Qiagen、Roche、sigma、Thermo、Zymo……等等品牌。

化工部主營:標(biāo)準(zhǔn)滴定溶液,PH緩沖液,指示劑等。

Elisa試劑盒,檢測試劑盒,進(jìn)口原裝Elisa試劑盒,抗體,培養(yǎng)基,生物試劑,細(xì)胞

 

Fetal Bovine Serum, Certified, US Origin

(Gibco®)

描述

 

 

 

Gibco® Sera: Trusted Quality for Consistent Performance

Sera Category: Performance Plus
• Use for a broad range of cell types, especially sensitive cell lines
• Our most characterized sera with the lowest endotoxin and hemoglobin levels
• Endotoxin level: ≤ 5 EU/ml
• Hemoglobin level: ≤ 10 mg/dl
• Origin: United States

Gibco® Serum Delivers
• ISO 13485 certified, processed in FDA registered facilities
• Labeled for in-vitro diagnostics (compliant with highest level of USP sterility testing)
• Triple filtered at 0.1 µm
• Gibco® bottle is easier to use in the hood, reduces the risk of contamination and helps you perform cell culture more consistently
• Peel-off sticker on label provides handy reference for lot number and expiration date recording in lab notebook

詳細(xì)說明

常用規(guī)格

Age:

Fetal 

Format:

Bottle(s) 

Species:

Bovine 

Product Size:

500 ml 

Endotoxin Level:

≤ 5 EU/ml 

Serum Treatment:

Standard (Sterile-Filtered) 

Hemoglobin Level:

≤ 10mg⁄dl 

Country of Origin:

United States 

Purity or Quality Grade:

Certified 

Shipping Condition:

 

額外信息

 

引文和參考文獻(xiàn) (10)

  • Raloxifene improves endothelial dysfunction in hypertension by reduced oxidative stress and enhanced nitric oxide production.

作者
Wassmann Sven; Laufs Ulrich; Stamenkovic Djordje; Linz Wolfgang; Stasch Johannes-Peter; Ahlbory Katja; Rösen Renate; Böhm Michael; Nickenig Georg;
雜志
Circulation (2002) 105:2083-2091
產(chǎn)品用途
This paper references Gibco cell culture media including FCS (same as FBS) for use in Vascular smooth muscle cells (VSMCs) from rat thoracic aorta. All antibiotics used were also from Gibco (Invitrogen). MMLV Reverse transcriptase was used on total RNA purified from the thoracic aorta cells to generate cDNA (60 minutes at 42 C and then 10 minutes at 75 C). 
ID:
PN61105
  • A 2-D liquid separations/mass mapping method for interlysate comparison of ovarian cancers.

作者
Kachman Maureen T; Wang Haixing; Schwartz Donald R; Cho Kathleen R; Lubman David M;
雜志
Anal Chem (2002) 74:1779-1791
產(chǎn)品用途
All cell culture components except where otherwise note are from Gibco® (Invitrogen). Human ovarian clear cell adenocarcinoma cells (ES-2) from ATCC were cultured in McCoy's 5A medium. Immortalized ovarian surface epithelial (OSE) cells were cultured in DMEM. Both lines were supplimented with 10% FBS and 100 U/mL penicillin and 100 ug/mL streptomycin. PBS was used to wash the cells before assaying. 
ID:
PN64708
  • Ascorbic-acid transporter Slc23a1 is essential for vitamin C transport into the brain and for perinatal survival.

作者
Sotiriou Sotiria; Gispert Suzana; Cheng Jun; Wang Yaohui; Chen Amy; Hoogstraten-Miller Shelley; Miller Georgina F; Kwon Oran; Levine Mark; Guttentag Susan H; Nussbaum Robert L;
雜志
Nat Med (2002) 8:514-521
產(chǎn)品用途
Trizol was used to prepare total RNA from mouse brain tissue. This total RNa was then treated with Amplification grade DNase I (18068-015) to remove DNA contamination without damaging the RNA. Primary mouse embryonic fibroblasts were cultured in DMEM (Gibco) supplimented with 15% FBS, 4mM L-glutamine (both Gibco and 1% non-essential amino acids and 1% v/v penicillin/streptomycin. 
ID:
PN61532
  • Dystrophin deficiency markedly increases enterovirus-induced cardiomyopathy: a genetic predisposition to viral heart disease.

作者
Xiong Dingding; Lee Gil-Hwan; Badorff Cornel; Dorner Andrea; Lee Sang; Wolf Paul; Knowlton Kirk U;
雜志
Nat Med (2002) 8:872-877
產(chǎn)品用途
DMEM was used with homogenized heart tissues in the initial steps of determining the CVB3 viral titer in the mouse hearts. The researchers grew Hela cells in DMEM containing 10% FBS and ChoK1 cells (from ATCC) in F-12K medium containing 2 mM L-glutamine and 10% FBS.  
ID:
PN61045
  • Fibroblast growth factor receptor-1 signaling induces osteopontin expression and vascular smooth muscle cell-dependent adventitial fibroblast migration in vitro.

作者
Li Guohong; Oparil Suzanne; Kelpke Stacey S; Chen Yiu-Fai; Thompson John A;
雜志
Circulation (2002) 106:854-859
產(chǎn)品用途
Primary cultures of RASMCs and AFs, derived from Sprugue-Dawley rats (Charles River), were cultured in DMEM supplimented with 10% FBS, 4 mmol/L L-glutamine, 100 U/mL penicillin and 100 ug/mL streptomycin. 
ID:
PN64874
  • Cell docking and on-chip monitoring of cellular reactions with a controlled concentration gradient on a microfluidic device.

作者
Yang Mengsu; Li Cheuk-Wing; Yang Jun;
雜志
Anal Chem (2002) 74:3991-4001
產(chǎn)品用途
Suspension HL-60 cells (human promyelocytic leukemia) were cultured in flasks using RPMI-1640 with L-glutamine, 10% FBS and 1% penicillin-streptomycin. HBSS (Hanks balanced salt solution) was used for cell loading and on chip experiments. The authors have used the ATP-dependent calcium uptake reaction of HL-60 cells as a model for on-chip measurement of the threshold ATP concentration that induces significant intracellular calcium signal.
ID:
PN47302
  • Ultraviolet-induced junD activation and apoptosis in myeloblastic leukemia ML-1 cells.

作者
Li T, Dai W, Lu L.
雜志
J Biol Chem (2002) 277:32668-32744
產(chǎn)品用途
Human myeloblastic leukemia ML-1 cells were grown in RPMI 1640 media with 7.5% FBS.
ID:
PN66040
  • Role for 18:1 lysophosphatidic acid as an autocrine mediator in prostate cancer cells.

作者
Xie Y, Gibbs TC, Mukhin YV, Meier KE.
雜志
J Biol Chem (2002) 277:32516-32542
產(chǎn)品用途
PC3 cells were grown in F12K media and Du145 cells were grown in RPMI 1640. Both media were supplemented with 10% FBS.
ID:
PN47584
  • The transcriptional regulation of human aldehyde dehydrogenase I gene. The structural and functional analysis of the promoter.

作者
Yanagawa Y; Chen J C; Hsu L C; Yoshida A;
雜志
J Biol Chem (1995) 270:17521-17528
產(chǎn)品用途
Transient transfection of Hep3B and LTK (mouse fibroblast) cells with plasmid DNA using Lipofectin.
ID:
PN65100
  • Influence of second and third cytoplasmic loops on binding, internalization, and coupling of chimeric bombesin/m3 muscarinic receptors.

作者
Tseng M J; Coon S; Stuenkel E; Struk V; Logsdon C D;
雜志
J Biol Chem (1995) 270:17884-17975
產(chǎn)品用途
Chimeric bombesin (Bn) receptors possessing cytoplasmic domains from the m3 muscarinic cholinergic ACh receptor were expressed in CHO-K1 cells. CHO-K1 cells were grown to 30-40% confluence in 60-mm dishes and transfected with 3 µg of PvuI-linearized plasmid DNA using Lipofectin reagent for 6-8 hours in serum-free medium. Cells then returned to 10% FBS, grown 36 h, and removed from the dishes by trypsin-EDTA treatment. Replated at reduced density in 150-mm plates with 1 mg/ml G418 (Geneticin).
ID:
PN63149

按細(xì)胞系排列的引文

細(xì)胞系名稱 有機(jī)體類型 組織
HL-60 Human Lymphocyte
CHO K1 Hamster Ovary
PC-3 Human Prostate
A6 Frog Kidney
LTK Mouse Connective
RCCD1 Rat Kidney
m-IMCD Mouse Kidney
HeLa cells Human Cervix

 

Dry Ice 

 

 

 
 
 
主營:生化試劑、抗體、重組蛋白、細(xì)胞生物、分子生物、蛋白研究、微生物、Elisa試劑盒、耗材、標(biāo)準(zhǔn)品、 標(biāo)準(zhǔn)溶液……
abcam、ABI、Axygen、corning、信號轉(zhuǎn)導(dǎo)抗體、Gibco、Hyclone、invitrogen、merck、Millipore、Qiagen、R&D、Roche、santa、sigma、Thermo等進(jìn)口品牌……
以下為本公司常備現(xiàn)貨產(chǎn)品

 

 

 

*部分 invitrogen 原裝產(chǎn)品

 

 

品牌 貨號 名稱 規(guī)格
invitrogen 13778-075 Lipofectamine RNAiMAX 0.75ml
invitrogen 13778-150 Lipofectamine RNAiMAX 1.5ml
invitrogen 11668-027 Lipofectamine 2000 Reagent 0.75ml
invitrogen 11668-019 Lipofectamine 2000 Reagent 1.5ml
invitrogen 15596-026 TRlzol ®Reagent 100ml
invitrogen 15596-018 TRlzol ®Reagent 200ml
invitrogen 10296-010 Trizol ®  LS Reagent 100ml
invitrogen 10296-028 Trizol ®  LS Reagent 200ml
invitrogen 17504-044 B-27(黃顏色) 10ml
invitrogen 12587-010 B-27(黃顏色)不含維生素A 10ml
invitrogen 17502-048 N-2  (粉紅顏色) 5ml
invitrogen 18064-014 SuperScript® II Reverse Transcriptase 10,000U
invitrogen 18080-044 SuperScript® III Reverse Transcriptase 10,000U

 

 

第二部分 HyClone胎牛血清

 

 

品牌 貨號 名稱 規(guī)格
HyClone SH30370.03 Australian Standard Fetal Bovine Serum 500ml
HyClone SH30406.02 FOETAL BOVINE SERUM 500ml
HyClone SH30401.01 New Zealand Calf Serum 500ml
HyClone SH30066.02 FetalClone® II 100ml
HyClone SH30088.03 STANDARD FETAL BOVINE SERUM 500ml
HyClone SH30118.02 Newborn Bovine Calf Serum, U.S. Origin 100ml
HyClone SH30070.02 FETAL BOVINE SERUM 100ml
HyClone SH30087.02 COSMIC CALF® SERUM 100ml
HyClone SH30070.03E FETALBOVINESERUM CHARACTERIZSED 500ml
100ml
HyClone SH30070.03 FETAL BOVINE SERUM 500ml
HyClone SH30080.03 FetalClone® I 500ml
HyClone SH30084.03 FOETAL BOVINE SERUM: CERTIFIED AUSTRALIAN 500ml
HyClone SH30396.03 FETAL BOVINE SERUM CHARACTERIZED 500ml
HyClone SV30087.02 南美源胎牛血清 500ml
HyClone sv30087.01 南美源胎牛血清 100ml
HyClone SH30071.03 FETAL BOVINE SERUM 500ml
HyClone SH30071.02 FETAL BOVINE SERUM 100ml
HyClone SH30080.02 FetalClone® I 100ml
HyClone SH30070.02E FETAL BOVINE SERUM CHARACTERIZSED 100ml
HyClone SH30079.02 FETAL BOVINE SERUM 100ml
HyClone SH30068.02 CHARCOAL/DEXTRAN TREATED FBS,
活性炭滅活血清
100ml
HyClone SH30068.03 CHARCOAL/DEXTRAN TREATED FBS,
活性炭滅活血清
500ml
HyClone SH30066.03 FetalClone® II 500ml
HyClone SH30109.03 HyClone FetalClone® III 500ml
HyClone SH30088.03 STANDARD FETAL BOVINE SERUM 500ml
HyClone SH30088.02 STANDARD FETAL BOVINE SERUM 100ml
HyClone SH30079.03 FETAL BOVINE SERUM 500ml

 

 

第二部分  Gibco胎牛血清

 

 

品牌 貨號 名稱 規(guī)格
Gibco 12662-029 MSC QUALIFIED FBS 500ML 500ML
Gibco 10438-026 FBS, HI 500ML 500ML 
Gibco 10439-024 FBS, ES CELL QUAL. USDA APPD. 500ML 500ML
Gibco 10099-141 FBS, AUSTRALIAN ORIGIN 500ML 500ML 
Gibco 26400-044 FBS, DIALYZED, USA ORIGIN 500ML 500ML
Gibco 26140-079 FBS QUALIFIED, USA ORIGIN 500ML 500ML
Gibco 16141-079 FBS, ES CELL QUAL, USA ORIGIN 500ML 500ML
Gibco 16140-071 FBS, HI 500ML 500ML
Gibco 16000-044 FBS, CERT, USA ORIGIN 500ML 500ML
Gibco C2027050   FBS, URUGUAYAN ORIGIN  500ML
Gibco 12657-029 巴西胎牛 500ml
 

 



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