Description :
Peptide NGlycosidase F, also known as PNGase F, is an amidase specifically remove asparaginelinked (Nlinked) polysaccharides from glycoproteins.
Applications :
1. proteomic analysis: MALDITOF mass spectrometry? identification of Nlinked glycosylation sites.
2. facilitate crystallization of glycoproteins.
Specifications :
Properties:
Biological Source: Recombinant PNGase F is a glycosidase cloned from Elizabethkingia miricola (formerly Flavobacterium meningosepticum ) and expressed in E. coli .
Concentration: 10,000 units/ml. Molecular Weight: PNGase F has a molecular weight of approximay 36 kDa. Physical Form: PNGase F is supplied in 20 mM TrisHCl (pH 7.5 at 25°C), 50 mM NaCl, and 5mM EDTA. Storage: Stored at 4-8°C.
Unit Definition: One unit of PNGase F will catalyze the deglycosylation of 1 nanomole of denatured Ribonuclease B (RNase B) in one minute at 37°C. One unit is equal to 1 IUB milliunit.

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